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DilutionCalculator
Disinfection, Cleaning & Household

Alcohol Dilution Calculator

Dilute alcohol. It allows for the small drop in volume.

Presets:
Formula: C₁ × V₁ = C₂ × V₂
V₁ = (C₂ × V₂) / C₁
Stock Source
Calculating
Target Conc
Final Volume
Fluid color:
Dilution Factor (DF)
5.00×
1 : 5 dilution
Solvent (Buffer / Water)
40.00 mL
V₂ - V₁ volume

Alcohol Dilution Calculator: Live Flask Simulation

Live color sync
Transfer 10 mL aliquot
Stock Solution (C₁)
75%50%25%
10 mM
Concentration (C₁)
Aliquot to draw: 10 mL
Final Dilution (C₂)
CALIBRATION MARK (V₂)
2 mM
Concentration (C₂)
Total Volume: 50 mL
Color depth (Beer-Lambert) Relative Intensity: 20% (Absorbance Factor: 0.200)

Step-by-Step Bench Protocol

1

Pipette Stock Aliquot

Draw 10 mL of stock (10 mM) with a pipette.

2

Add Diluent / Buffer

Add 40 mL of water or buffer to reach the final mark.

3

Invert and Mix

Invert the tube to mix. You now have 50 mL at 2 mM (a 5× dilution).

How It Works

This calculator works in 4 steps.

  1. Pick the alcohol and basis

    Select ethanol or isopropyl alcohol and % v/v or % w/w.

  2. Enter the stock strength

    Type the strength on the label.

  3. Enter the target and final volume

    Type the target % v/v and the final volume.

  4. Read the volumes

    The calculator returns alcohol volume and the instruction to fill to the final mark with water.

The Formula

The formula applies C₁V₁ = C₂V₂ in % v/v and fixes the final volume after mixing.

Alcohol volume V_alc = %_target × V_final / %_stock
Water (estimate only) V_water > V_final − V_alc (contraction)
w/w to v/v %v/v = %w/w × ρ_solution / ρ_alcohol
%_stock / %_target:
Stock and target strength (% v/v)
V_final:
Final volume after mixing
ρ:
Densities at 20 °C
Worked example

1 L of 70% v/v isopropyl alcohol from 99% stock needs 707.1 mL of alcohol, with water added to the 1,000 mL mark.

Conversion Table

The conversion table gives the alcohol volume for 1 L (33.8 US fl oz) of 6 common target strengths.

AlcoholStockTargetAlcohol per 1 LWater step
Ethanol95% v/v70% v/v736.8 mLWater to 1,000 mL
Ethanol99.5% v/v70% v/v703.5 mLWater to 1,000 mL
Ethanol95% v/v80% v/v842.1 mLWater to 1,000 mL
Isopropyl alcohol99% v/v70% v/v707.1 mLWater to 1,000 mL
Isopropyl alcohol91% v/v70% v/v769.2 mLWater to 1,000 mL
Ethanol70% v/v50% v/v714.3 mLWater to 1,000 mL

What Is the Alcohol Dilution Calculator?

The Alcohol Dilution Calculator is a tool that dilutes ethanol or isopropyl alcohol to a target strength, accounting for the concentration basis (% v/v or % w/w) and for volume contraction on mixing. The calculator returns the alcohol volume and a make-to-volume instruction.

Alcohol and water shrink when mixed, so adding 26.3 mL of water to 73.7 mL of 95% ethanol gives less than 100 mL. Measuring the alcohol and filling to the final mark with water gives the correct % v/v. Labels for spirits and solvents differ in basis, so the calculator asks whether the stock is % v/v or % w/w and converts with density tables.

Common Uses

There are 5 main uses of this calculator.

  • Surface disinfection

    Labs and clinics prepare 70% alcohol for wiping surfaces.

  • Lab washes

    Molecular labs prepare 70% and 80% ethanol for DNA washes.

  • Electronics cleaning

    Technicians dilute isopropyl alcohol for board cleaning.

  • Extraction solvents

    Herbal and lab extractions use set alcohol strengths.

  • Spirits blending

    Distillers proof spirits down to bottling strength.

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