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Every calculator, guide and help page on CalculatorDilution.com, grouped by topic.
Main Tools
Solve any dilution and watch the flask fill in real time.
Browse all 158 dilution tools by topic.
Find C₁, V₁, C₂ or V₂ from the other three values.
Plan tube-by-tube series for ELISA, qPCR and MIC tests.
Formulas, safety rules and dilution factor math.
Core C₁V₁ & Volume Solvers
Solve for any one of the four values. Type the other three.
Find how much stock to use. Then top up to the final mark.
Find the strength after you dilute. Enter stock and volumes.
Find the stock strength you need. Work back from the target.
Find the total volume that hits your target. Use what stock you have.
Find how much water or buffer to add. Volumes are taken to add up.
Find the stock to add, then fill to a set final volume. Good for flasks with a fill line.
Find the stock to add to a set amount of diluent. Use it when the buffer is pre-filled.
Dilution Factors & Ratios
Find how many times weaker the final mix is than the stock. A factor of 10 means ten times weaker.
Turn a mix ratio into real volumes. You set what the ratio means.
Show stock as parts of the water or buffer you add. 1:4 here means 1 part stock to 4 parts water.
Show stock as parts of the whole final mix. 1:5 here means 1 part stock in 5 parts total.
Switch between the two ways labs write a ratio. It stops 1:4 and 1:5 mix-ups.
Turn a dilution factor into a ratio, and back. Pick which ratio style you use.
Work with x-fold steps, such as 10-fold or 2-fold. See the new strength at each fold.
Flip a dilution such as 1/100 into its factor of 100. It also reads 10⁻³ style input.
Find the first strength from a reading on a diluted sample. Multiply the reading by the factor.
Fix a result when you diluted the sample by hand first. It adds the machine's own step too.
Multiply each step to get the full dilution factor. Two 1:10 steps make 1:100.
Serial & Step Dilution
Find the strength in each tube of a series. Set the start, the factor and the tube count.
Halve the strength at each step. See every tube.
Cut the strength ten times at each step. See every tube.
Use the same factor you pick at every step. Try 3-fold, 4-fold or any factor.
Use a new factor at each step of the series. Mix 10-fold and 2-fold steps in one series.
Find how many steps it takes to reach your target. It rounds up to whole steps.
Find the step factor that links your first and last tube. Set the count of tubes you want.
Find how much to move from tube to tube. Each tube ends with the same volume.
Find the water or buffer for each tube. Fill each tube first, then transfer.
See how much is left in each tube after you move some on. The last tube keeps its full volume.
Find how much stock the whole series uses. Add a bit extra for the pipette.
Add up the sample and buffer for the whole series. Order enough before you start.
Multi-Stage & Series Planning
Add a middle step when the stock is too strong to use. It keeps each transfer easy to pipette.
Split one big dilution into steps you can pipette. Each step stays in a safe range.
Compare one big step with a series. See which uses less.
Make each strength straight from the stock, not from the last tube. An error in one tube stays in that tube.
Make a series where each step is the same times smaller. Good for dose curves on a log scale.
Make a series with even gaps between each strength. Good for straight-line standard curves.
Plan the volumes for each point on a standard curve. Each point gets its own stock and buffer volume.
Work back to each standard's true strength from its steps. It follows each step from the stock.
Concentration Units & Chemistry
Dilute in M, mM, µM or nM. Mix units between stock and target.
Dilute in mg/mL, g/L and other mass units. Mix units between stock and target.
Dilute in % w/v, which is grams per 100 mL. So 5% w/v is 5 g in 100 mL.
Dilute in % v/v, which is mL per 100 mL. So 70% v/v is 70 mL in 100 mL.
Dilute in % w/w by weight, not volume. Weigh each part on a scale.
Dilute in mol/kg. It uses the mass of the solvent.
Dilute in N. You set what counts as one unit.
Dilute in parts per million. Pick a mass or volume base.
Dilute in parts per billion for trace work. One ppb is one part in a billion.
Dilute in parts per trillion. Pick the base you need.
Work with strengths like 1:1,000 as used in drug labels. 1:1,000 means 1 g in 1,000 mL.
Switch between strength units that match. It will not mix mass and mole units.
Switch between M, mM, µM and nM. Each step is 1,000 times.
Switch between g/L, mg/mL, µg/mL and more. Note that 1 mg/mL is 1 g/L.
Turn M into g/L with the molar mass, and back. Type the molar mass from the label.
Turn % into ppm, and back. One % is 10,000 ppm.
Turn % by weight into a per-volume strength. It needs the density.
Turn % by volume into % by weight with each density.
Turn the weight of a liquid into its volume with density.
Switch between L, mL, µL, cups, gallons and more.
Stock Reagent & Reconstitution
Find how much to weigh to make a stock of set strength.
Find the grams of powder you need to weigh.
Weigh a bit more when the powder is not pure.
Use the right weight when the salt holds water.
Find how much liquid to add to a dry vial.
Add the right liquid to a freeze-dried vial.
Find the M of a bottle acid from its label.
Solution Mixing & Buffer Systems
Mix a strong and a weak stock to hit a target.
Find how much of each stock to mix for your target.
Track each part of a mix on its own.
Use a diluent that already holds some of the solute.
Keep the same base mix in every sample.
Use when the final volume is less than the sum of parts.
Track the % of each solvent in the final mix.
Find how much solvent the stock brings into the mix.
Check that a solvent such as DMSO stays under your limit.
Find the final strength of each part of a buffer.
Turn a 10x or 5x buffer into 1x.
Dilute an acid or base. Always add acid to water.
Check that no solute is lost or gained along the way.
Biomolecule, Cell & Assay Dilutions
Dilute a protein stock to the strength you need.
Turn an antibody ratio into antibody and buffer volumes.
Scale a primary antibody ratio to your blots or slides.
Scale a secondary antibody ratio to your read step.
Dilute DNA to the ng/µL you need.
Find the RNA and water to mix for each sample.
Add the right water to a dry oligo to make a stock.
Make primer stocks for a PCR run.
Mix two or more primers into one tube.
Bring all samples to the same strength.
Load the same amount from each sample.
Dilute cells to the count per mL you need.
Count only live cells when you dilute.
Find how much cell mix to add to each well or flask.
Dilute a culture to the start point you need.
Turn plate counts back into CFU per mL.
Fix a cell count when you diluted before you counted.
Dilute a stain to the strength in your protocol.
Dilute an enzyme to the units per mL you need.
Make working strengths from a compound stock.
Make a reagent strong enough for the dilution in the well.
Find the new strength after you add a spike.
Keep the same solvent % in every well.
Microplate & Liquid Handling
Plan the volume for each well on a plate.
Run a series across the rows of a plate.
Run a series down the columns of a plate.
Scale up when you run each sample more than once.
Cross two series on one plate.
Keep each step in the range your pipette can hold.
Round to what your pipette can set. See the new strength.
Add a middle step when a volume is too small to pipette.
Add the extra that stays stuck in tubes and tips.
Make a bit more than you need so you do not run short.
Split one batch into small tubes of equal size.
Plan your steps when you have only a little sample.
Quality Control, Error & Uncertainty
Check that the batch fits in your tube or bottle.
See how small volume errors change the final strength.
See how error grows down a series.
Compare what you measured with what you expected.
Disinfection, Cleaning & Household
Undo every dilution step to get the first value.
Mix a cleaner at the ratio on its label.
Mix bleach and water the way the label says.
Mix a disinfectant the way the label says.
Scale a proven hand rub recipe up or down.
Dilute alcohol. It allows for the small drop in volume.
Dilute peroxide to the % you need.
Find how much soap and water to mix.
Fit a label ratio to the size of your spray bottle.
Fit a label ratio to the size of your bucket.
Mix soap for a foam cannon. It counts the water the hose adds.
Find the final strength when an injector draws the mix.
Mix a premix, then let the gear dilute it again.
Specialty, Agriculture & Industry
Mix pool chemicals the way the label says.
Dilute plant food stock for your tank.
Mix plant food at the rate on its label.
Fill a spray tank at the rate on the label.
Thin paint at the ratio the maker gives.
Mix paint, hardener and thinner in the right parts.
Mix an oil with a carrier at the % you pick.
Add water to wort and see the new gravity.
See the new ABV after you add water.
Dilute salt water to the % you need.
Generators, Reports & Batch Tools
Dilute a sugar syrup by weight or by Brix.
Find the cost of each mixed batch.
See how much ready mix your stock will make.
Make a chart of ratios, volumes and strengths.
Draw a series as tubes with arrows.
Make practice sheets with answers for a class.
Print labels with the strength, date and name.
Work out many dilutions at once.
Check your numbers for mistakes before you mix.
Save a full record of a dilution as one file.
Make a sheet you can reuse for each batch.
Financial & Equity Dilution
See your new stake after the firm sells new shares.
See how a funding round changes who owns what.
See how a larger option pool cuts each stake.
See what happens when notes or SAFEs turn into shares.
Count every share, plus all that could become shares.
Find diluted EPS under the rules you pick.
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