How It Works
This calculator works in 3 steps.
Enter the supplied amount
Type the mass on the vial label in µg, mg, or g.
Enter the target concentration
Type the stock strength, such as 1 mg/mL.
Read the solvent volume
The calculator returns the volume in mL and µL.
Check the vial capacity
Compare the volume with the vial size before adding solvent.
The Formula
The formula divides the supplied amount by the target concentration.
V = m / CV = n / M, where n = m / MW- V:
- Solvent volume
- m:
- Supplied mass
- C:
- Target mass concentration
- n / M:
- Moles supplied and target molarity
A 100 µg vial reconstituted to 0.1 mg/mL (100 µg/mL) needs 100 / 100 = 1 mL of solvent.
Conversion Table
The conversion table gives solvent volumes for 6 supplied amounts and target concentrations.
| Supplied amount | Target | Solvent (mL) | Solvent (µL) |
|---|---|---|---|
| 1 mg | 1 mg/mL | 1 mL | 1,000 µL |
| 5 mg | 2 mg/mL | 2.5 mL | 2,500 µL |
| 10 mg | 10 mg/mL | 1 mL | 1,000 µL |
| 100 µg | 0.1 mg/mL | 1 mL | 1,000 µL |
| 50 µg | 0.5 mg/mL | 0.1 mL | 100 µL |
| 2 mg | 0.25 mg/mL | 8 mL | 8,000 µL |
What Is the Reconstitution Volume Calculator?
The Reconstitution Volume Calculator is a tool that returns the volume of solvent to add to a supplied amount of reagent to reach a target concentration. The volume equals the supplied amount divided by the target concentration.
Suppliers ship proteins, antibodies, and small molecules as fixed amounts, such as 100 µg or 5 mg. The calculator turns that amount into a solvent volume for a chosen stock concentration. The tool assumes the dissolved solid adds negligible volume, which holds for amounts below about 10 mg/mL.
Common Uses
There are 5 main uses of this calculator.
Recombinant protein vials
Users reconstitute 10 µg to 1 mg protein vials.
Antibody stocks
Researchers reconstitute lyophilized antibodies to 0.5 or 1 mg/mL.
Small-molecule powders
Pharmacologists dissolve 5 mg inhibitor vials into DMSO stocks.
Cytokines
Cell biologists reconstitute cytokines to 100 µg/mL.
Reference standards
Analysts dissolve pre-weighed standards to a set strength.