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DilutionCalculator
Generators, Reports & Batch Tools

Serial Dilution Diagram Generator

Draw a series as tubes with arrows.

Presets:
Formula: C₁ × V₁ = C₂ × V₂
V₁ = (C₂ × V₂) / C₁
Stock Source
Calculating
Target Conc
Final Volume
Fluid color:
Dilution Factor (DF)
5.00×
1 : 5 dilution
Solvent (Buffer / Water)
40.00 mL
V₂ - V₁ volume

Serial Dilution Diagram Generator: Live Flask Simulation

Live color sync
Transfer 10 mL aliquot
Stock Solution (C₁)
75%50%25%
10 mM
Concentration (C₁)
Aliquot to draw: 10 mL
Final Dilution (C₂)
CALIBRATION MARK (V₂)
2 mM
Concentration (C₂)
Total Volume: 50 mL
Color depth (Beer-Lambert) Relative Intensity: 20% (Absorbance Factor: 0.200)

Step-by-Step Bench Protocol

1

Pipette Stock Aliquot

Draw 10 mL of stock (10 mM) with a pipette.

2

Add Diluent / Buffer

Add 40 mL of water or buffer to reach the final mark.

3

Invert and Mix

Invert the tube to mix. You now have 50 mL at 2 mM (a 5× dilution).

How It Works

This generator works in 4 steps.

  1. Enter the series parameters

    Type the starting concentration, factor, transfer volume, and tube count.

  2. Choose the layout

    Select a horizontal row, a vertical column, or a plate view.

  3. Style the labels

    Show or hide volumes, concentrations, DF, and exponent notation.

  4. Export the diagram

    Download SVG or PNG, or copy it to the clipboard.

The Formula

The formula labels each tube with the starting concentration divided by the factor raised to the tube number.

Tube label Cₙ = C₀ / Fⁿ
Arrow label V_t = V_d / (F − 1)
Color scale shade ∝ log₁₀(Cₙ)
C₀:
Starting concentration
F:
Factor per step
V_t / V_d:
Transfer and diluent volumes
Worked example

A tenfold series from 1,000 µg/mL with 100 µL into 900 µL draws 5 tubes labeled 100, 10, 1, 0.1, and 0.01 µg/mL.

Conversion Table

The conversion table lists the labels the generator places on a 5-tube tenfold diagram from 1,000 µg/mL.

PositionArrow labelDiluentTube labelFlow
Stock——1,000 µg/mLSource
Tube 1100 µL from previous900 µL100 µg/mL→ next tube
Tube 2100 µL from previous900 µL10 µg/mL→ next tube
Tube 3100 µL from previous900 µL1 µg/mL→ next tube
Tube 4100 µL from previous900 µL0.1 µg/mL→ next tube
Tube 5100 µL from previous900 µL0.01 µg/mLEnd

What Is the Serial Dilution Diagram Generator?

The Serial Dilution Diagram Generator is a tool that draws a labeled diagram of a serial dilution, with tubes, transfer arrows, volumes, and the concentration in each tube. The diagram updates as the user changes the factor, volume, or tube count.

A diagram shows the transfer path at a glance, so it suits bench sheets, lab reports, and teaching slides. The generator colors tubes by concentration, labels each arrow with the transfer volume, and exports SVG for editing or PNG for documents.

Common Uses

There are 5 main uses of this generator.

  • Lab reports

    Students add method diagrams to reports.

  • Teaching slides

    Instructors show serial dilution steps in lectures.

  • Bench sheets

    Technicians print the diagram above the tube rack.

  • SOP figures

    QA teams add standard diagrams to SOPs.

  • Grant and paper figures

    Researchers prepare method schematics.

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