How It Works
This planner works in 4 steps.
Enter the row A concentration
Type the top concentration for each column.
Choose the factor and rows
Type the step factor and the number of dilution rows, such as 7 plus 1 blank.
Set the well volume
Type the final volume per well.
Read the plan
The planner returns pre-fill, transfer, and concentration for rows A to H.
The Formula
The formula divides the row A concentration by the factor raised to the row index.
C_row = C_A / F^(r)r = 0 for A, 1 for B … 7 for HV_t = V_well / (F − 1)- C_A:
- Concentration in row A
- F:
- Step factor
- r:
- Row index
- V_well:
- Final well volume
A threefold column from 1,000 ng/mL with 100 µL per well transfers 50 µL into 100 µL pre-fill and ends at 1.37 ng/mL in row G, with row H as blank.
Conversion Table
The conversion table shows a threefold column from 1,000 ng/mL with row H as the blank.
| Row | Concentration (ng/mL) | Cumulative DF | Final volume |
|---|---|---|---|
| A | 1,000 | 1 | 100 µL |
| B | 333.3 | 3 | 100 µL |
| C | 111.1 | 9 | 100 µL |
| D | 37.04 | 27 | 100 µL |
| E | 12.35 | 81 | 100 µL |
| F | 4.115 | 243 | 100 µL |
| G | 1.372 | 729 | 100 µL |
| H | 0 (blank) | — | 100 µL |
What Is the Plate Column Dilution Planner?
The Plate Column Dilution Planner is a tool that arranges a serial dilution vertically down a plate column, from row A to row H in a 96-well plate, and returns the transfer plan and concentration per row. Each column holds one sample, so 12 columns hold 12 series.
Column layouts give 8 levels per series, which matches common 7-point standard curves plus a blank. An 8-channel pipette fits a column, so column series are made with a single-channel pipette row by row, or prepared in tubes and transferred with a multichannel. The planner returns both options.
Common Uses
There are 5 main uses of this planner.
ELISA standards
Users run 7 standards plus a blank down columns 1 and 2.
12-sample titrations
Labs titrate 12 sera, 1 per column.
BCA and Bradford standards
Biochemists load protein standards down a column.
Hemagglutination assays
Virologists run 12 samples in V-bottom columns.
Checkerboard axes
Researchers make the vertical axis of a 2-drug grid.