How It Works
This planner works in 4 steps.
Enter the top concentration
Type the column 1 concentration for each row.
Choose the factor and columns
Type the step factor and the number of dilution columns, such as 11 plus 1 control.
Set the well volume
Type the final volume per well.
Read the plan
The planner returns pre-fill, transfer, and discard volumes and each column's concentration.
The Formula
The formula divides the column 1 concentration by the factor raised to the column index.
C_col = C₁ / F^(col − 1)V_t = V_well / (F − 1)V_top = V_well + V_t- C₁:
- Concentration in column 1
- F:
- Step factor
- col:
- Column number
- V_well:
- Final well volume
A twofold row from 100 µM with 100 µL per well uses 200 µL in column 1 and 100 µL diluent in columns 2 to 11. Transfer 100 µL left to right and discard 100 µL from column 11. Column 11 holds 0.0977 µM.
Conversion Table
The conversion table shows a 12-column twofold row from 100 µM with 100 µL per well and column 12 as the control.
| Column | Concentration (µM) | Transfer | Final volume | Cumulative DF |
|---|---|---|---|---|
| 1 | 100 | 200 µL top | 100 µL | 1 |
| 2 | 50 | 100 µL from previous | 100 µL | 2 |
| 3 | 25 | 100 µL from previous | 100 µL | 4 |
| 4 | 12.5 | 100 µL from previous | 100 µL | 8 |
| 5 | 6.25 | 100 µL from previous | 100 µL | 16 |
| 6 | 3.125 | 100 µL from previous | 100 µL | 32 |
| 7 | 1.562 | 100 µL from previous | 100 µL | 64 |
| 8 | 0.7812 | 100 µL from previous | 100 µL | 128 |
| 9 | 0.3906 | 100 µL from previous | 100 µL | 256 |
| 10 | 0.1953 | 100 µL from previous | 100 µL | 512 |
| 11 | 0.09766 | 100 µL from previous | 100 µL | 1,024 |
| 12 | 0 (control) | Diluent only | 100 µL | — |
What Is the Plate Row Dilution Planner?
The Plate Row Dilution Planner is a tool that arranges a serial dilution horizontally along a plate row, from column 1 to column 12 in a 96-well plate, and returns the transfer plan and concentration for each well. Each row holds one sample or compound, so 8 rows hold 8 series.
Row layouts fit 12-point dose-response curves and 11-point series with one control column. The planner states the top well volume, the diluent pre-fill for columns 2 to 11, the transfer volume, and the discard from the last dilution well. A multichannel pipette moves all 8 rows at once, column by column.
Common Uses
There are 5 main uses of this planner.
IC₅₀ curves
Pharmacologists run 8 compounds per plate, 1 per row.
Antibody titration
Users titrate 8 antibodies across 12 dilutions.
MIC rows
Microbiologists run 8 isolates against 1 antibiotic series.
Cytokine standards
Users run standard curves along row A.
Multichannel workflows
Technicians transfer 8 rows in 1 move per column.