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DilutionCalculator
Generators, Reports & Batch Tools

Batch Dilution Calculator

Work out many dilutions at once.

Presets:
Formula: C₁ × V₁ = C₂ × V₂
V₁ = (C₂ × V₂) / C₁
Stock Source
Calculating
Target Conc
Final Volume
Fluid color:
Dilution Factor (DF)
5.00×
1 : 5 dilution
Solvent (Buffer / Water)
40.00 mL
V₂ - V₁ volume

Batch Dilution Calculator: Live Flask Simulation

Live color sync
Transfer 10 mL aliquot
Stock Solution (C₁)
75%50%25%
10 mM
Concentration (C₁)
Aliquot to draw: 10 mL
Final Dilution (C₂)
CALIBRATION MARK (V₂)
2 mM
Concentration (C₂)
Total Volume: 50 mL
Color depth (Beer-Lambert) Relative Intensity: 20% (Absorbance Factor: 0.200)

Step-by-Step Bench Protocol

1

Pipette Stock Aliquot

Draw 10 mL of stock (10 mM) with a pipette.

2

Add Diluent / Buffer

Add 40 mL of water or buffer to reach the final mark.

3

Invert and Mix

Invert the tube to mix. You now have 50 mL at 2 mM (a 5× dilution).

How It Works

This calculator works in 4 steps.

  1. Paste or upload the records

    Paste rows from a spreadsheet or upload a CSV file.

  2. Map the columns

    Match columns to name, C₁, C₂, V₂, and units.

  3. Run the batch

    The calculator solves each row and flags errors.

  4. Export the results

    Download CSV or print a worklist with totals.

The Formula

The formula applies C₁V₁ = C₂V₂ to every row and sums volumes by material.

Row stock volume V₁ᵢ = C₂ᵢ × V₂ᵢ / C₁ᵢ
Row diluent V_dᵢ = V₂ᵢ − V₁ᵢ
Material total Σ V₁ᵢ for rows sharing a stock
i:
Row index
C₁ᵢ / C₂ᵢ:
Stock and target for row i
V₂ᵢ:
Final volume for row i
Worked example

A batch of 6 records returns stock volumes from 1 mL to 50 mL, with total diluent of 932 mL across all rows.

Conversion Table

The conversion table shows a 6-row sample batch with stock and diluent volumes per row (shared concentration unit per row).

RecordStock (C₁)Target (C₂)Final volumeStock volumeDiluent
Buffer A101500 mL50 mL450 mL
Buffer B51250 mL50 mL200 mL
Std 11,00050100 mL5 mL95 mL
Std 21,00010100 mL1 mL99 mL
Reagent X100250 mL1 mL49 mL
Reagent Y200.540 mL1 mL39 mL

What Is the Batch Dilution Calculator?

The Batch Dilution Calculator is a tool that processes many dilution records at once, from a pasted list or an uploaded CSV file, and returns stock and diluent volumes for every row. Each row carries its own stock, target, and volume.

Batch processing saves time when a day's work includes dozens of solutions. The calculator validates each row, flags impossible rows (target above stock), sums the total stock and diluent per material, and exports the result as CSV or a printable worklist.

Common Uses

There are 5 main uses of this calculator.

  • Daily preparation lists

    Technicians compute all solutions for the day at once.

  • Standard sets

    Analysts compute full calibration sets from one list.

  • LIMS exports

    Users process records exported from a LIMS.

  • Core facility orders

    Core labs process customer reagent orders.

  • Production schedules

    Plants compute batches across a production week.

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