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DilutionCalculator
Solution Mixing & Buffer Systems

Target Concentration Mixing Calculator

Find how much of each stock to mix for your target.

Presets:
Formula: C₁ × V₁ = C₂ × V₂
V₁ = (C₂ × V₂) / C₁
Stock Source
Calculating
Target Conc
Final Volume
Fluid color:
Dilution Factor (DF)
5.00×
1 : 5 dilution
Solvent (Buffer / Water)
40.00 mL
V₂ - V₁ volume

Target Concentration Mixing Calculator: Live Flask Simulation

Live color sync
Transfer 10 mL aliquot
Stock Solution (C₁)
75%50%25%
10 mM
Concentration (C₁)
Aliquot to draw: 10 mL
Final Dilution (C₂)
CALIBRATION MARK (V₂)
2 mM
Concentration (C₂)
Total Volume: 50 mL
Color depth (Beer-Lambert) Relative Intensity: 20% (Absorbance Factor: 0.200)

Step-by-Step Bench Protocol

1

Pipette Stock Aliquot

Draw 10 mL of stock (10 mM) with a pipette.

2

Add Diluent / Buffer

Add 40 mL of water or buffer to reach the final mark.

3

Invert and Mix

Invert the tube to mix. You now have 50 mL at 2 mM (a 5× dilution).

How It Works

This calculator works in 4 steps.

  1. Enter the high concentration

    Type the stronger solution's value.

  2. Enter the low concentration

    Type the weaker solution's value, or 0 for pure diluent.

  3. Enter the target and batch volume

    Type the target, which must fall between the 2 strengths, and the total volume.

  4. Read the parts and volumes

    The calculator returns parts high : parts low and the volume of each.

The Formula

The formula takes the parts of each solution from the differences between the target and the other solution.

Parts of high solution P_H = C_T − C_L
Parts of low solution P_L = C_H − C_T
Volume of high solution V_H = V_T × P_H / (P_H + P_L)
C_H / C_L:
High and low concentrations
C_T:
Target concentration
V_T:
Total batch volume
P_H / P_L:
Parts of each solution
Worked example

For 1,000 mL of 70% from 95% ethanol and water (0%), P_H = 70 and P_L = 25. V_H = 1,000 × 70 / 95 = 736.8 mL ethanol and 263.2 mL water, before contraction.

Conversion Table

The conversion table gives alligation parts and volumes for 6 strength combinations.

HighLowTargetParts (H : L)High volumeLow volume
70205030 : 20300 mL200 mL
9507070 : 25736.8 mL263.2 mL
10253 : 537.5 mL62.5 mL
30363 : 24111.1 mL888.9 mL
50102515 : 2575 mL125 mL
12033 : 9946.2 mL2,839 mL

What Is the Target Concentration Mixing Calculator?

The Target Concentration Mixing Calculator is a tool that returns the proportions of a strong and a weak solution needed to make a mixture at a target concentration in between. The calculator uses the alligation method (the mixing-cross method).

Alligation finds the parts of each solution by taking the distance from the target to the opposite strength. The weak solution can be plain water at 0%, which turns the method into a standard dilution. The calculator returns parts, fractions, and volumes for a chosen batch size.

Common Uses

There are 5 main uses of this calculator.

  • Pharmacy compounding

    Pharmacists mix 2 ointment or solution strengths to an ordered strength.

  • Alcohol strength

    Users blend 95% ethanol with 40% ethanol to reach 70%.

  • Nutrient blending

    Growers mix 2 nutrient tanks to a target EC.

  • Cleaning stock

    Facility staff blend concentrate and ready-to-use stock.

  • Syrup strength

    Food makers hit a target Brix from 2 syrups.

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