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DilutionCalculator
Solution Mixing & Buffer Systems

Two-Solution Mixing Calculator

Mix a strong and a weak stock to hit a target.

Presets:
Formula: C₁ × V₁ = C₂ × V₂
V₁ = (C₂ × V₂) / C₁
Stock Source
Calculating
Target Conc
Final Volume
Fluid color:
Dilution Factor (DF)
5.00×
1 : 5 dilution
Solvent (Buffer / Water)
40.00 mL
V₂ - V₁ volume

Two-Solution Mixing Calculator: Live Flask Simulation

Live color sync
Transfer 10 mL aliquot
Stock Solution (C₁)
75%50%25%
10 mM
Concentration (C₁)
Aliquot to draw: 10 mL
Final Dilution (C₂)
CALIBRATION MARK (V₂)
2 mM
Concentration (C₂)
Total Volume: 50 mL
Color depth (Beer-Lambert) Relative Intensity: 20% (Absorbance Factor: 0.200)

Step-by-Step Bench Protocol

1

Pipette Stock Aliquot

Draw 10 mL of stock (10 mM) with a pipette.

2

Add Diluent / Buffer

Add 40 mL of water or buffer to reach the final mark.

3

Invert and Mix

Invert the tube to mix. You now have 50 mL at 2 mM (a 5× dilution).

How It Works

This calculator works in 4 steps.

  1. Enter solution A

    Type concentration C_A and volume V_A.

  2. Enter solution B

    Type concentration C_B and volume V_B in the same units.

  3. Combine solute amounts

    The calculator computes C_A × V_A + C_B × V_B.

  4. Read the mixture concentration

    The calculator divides by V_A + V_B and returns C_mix and the total volume.

The Formula

The formula divides the total solute amount by the total volume.

Mixture concentration C_mix = (C_A × V_A + C_B × V_B) / (V_A + V_B)
Total volume V_mix = V_A + V_B
C_A / C_B:
Concentrations of solutions A and B
V_A / V_B:
Volumes of solutions A and B
C_mix:
Mixture concentration
Worked example

Mixing 300 mL of 70% ethanol with 200 mL of 20% ethanol gives (70 × 300 + 20 × 200) / 500 = 50% v/v, before volume contraction.

Conversion Table

The conversion table gives the mixture concentration for 6 pairs of solutions (concentrations in any shared unit).

Solution ASolution BTotal volumeMixture concentration
10 @ 50 mL2 @ 50 mL100 mL6
70 @ 300 mL20 @ 200 mL500 mL50
1 @ 100 mL0.1 @ 400 mL500 mL0.28
5 @ 25 mL0 @ 75 mL100 mL1.25
40 @ 1 mL10 @ 3 mL4 mL17.5
95 @ 500 mL50 @ 500 mL1,000 mL72.5

What Is the Two-Solution Mixing Calculator?

The Two-Solution Mixing Calculator is a tool that returns the concentration of a mixture made by combining 2 compatible solutions of the same solute at different concentrations. The result is the volume-weighted average of the 2 concentrations.

Mixing 2 strengths is common when using up partial bottles or blending a strong and a weak lot. The calculator adds the solute amounts from both solutions and divides by the combined volume. Both concentrations need the same unit, and the volumes are assumed additive.

Common Uses

There are 5 main uses of this calculator.

  • Partial bottle use

    Labs combine leftover stocks of different strengths.

  • Lot blending

    Producers blend a high and a low lot to hit a spec.

  • Buffer correction

    Technicians add stronger buffer to an over-diluted batch.

  • Alcohol blending

    Distillers blend spirits of 2 strengths.

  • Syrup blending

    Food makers blend syrups of different sugar content.

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