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DilutionCalculator
Concentration Units & Chemistry

Weight/Volume Percent Dilution Calculator

Dilute in % w/v, which is grams per 100 mL. So 5% w/v is 5 g in 100 mL.

Presets:
Formula: C₁ × V₁ = C₂ × V₂
V₁ = (C₂ × V₂) / C₁
Stock Source
Calculating
Target Conc
Final Volume
Fluid color:
Dilution Factor (DF)
5.00×
1 : 5 dilution
Solvent (Buffer / Water)
40.00 mL
V₂ - V₁ volume

Weight/Volume Percent Dilution Calculator: Live Flask Simulation

Live color sync
Transfer 10 mL aliquot
Stock Solution (C₁)
75%50%25%
10 mM
Concentration (C₁)
Aliquot to draw: 10 mL
Final Dilution (C₂)
CALIBRATION MARK (V₂)
2 mM
Concentration (C₂)
Total Volume: 50 mL
Color depth (Beer-Lambert) Relative Intensity: 20% (Absorbance Factor: 0.200)

Step-by-Step Bench Protocol

1

Pipette Stock Aliquot

Draw 10 mL of stock (10 mM) with a pipette.

2

Add Diluent / Buffer

Add 40 mL of water or buffer to reach the final mark.

3

Invert and Mix

Invert the tube to mix. You now have 50 mL at 2 mM (a 5× dilution).

How It Works

This calculator works in 4 steps.

  1. Enter the stock % w/v

    Type the percent on the stock label.

  2. Enter the target % w/v

    Type the working percent.

  3. Enter the final volume

    Type the total volume in mL or L.

  4. Read the stock volume

    The calculator returns V₁ and the grams of solute in the final volume.

The Formula

The formula applies C₁V₁ = C₂V₂ with % w/v as the concentration unit.

% w/v dilution %₁ × V₁ = %₂ × V₂
Definition % w/v = (g solute / mL solution) × 100
Conversion 1% w/v = 10 g/L = 10 mg/mL
%₁ / %₂:
Stock and target % w/v
V₁ / V₂:
Stock and final volumes
Worked example

To make 250 mL of 5% w/v glucose from 20% w/v stock, V₁ = 5 × 250 / 20 = 62.5 mL. The final solution holds 12.5 g glucose.

Conversion Table

The conversion table lists stock volumes and g/L equivalents for 6 % w/v dilutions.

StockTargetTarget (g/L)Final volumeStock volume
10% w/v1% w/v10 g/L100 mL10 mL
20% w/v5% w/v50 g/L250 mL62.5 mL
50% w/v0.9% w/v9 g/L500 mL9 mL
5% w/v0.5% w/v5 g/L1,000 mL100 mL
30% w/v3% w/v30 g/L200 mL20 mL
2% w/v0.1% w/v1 g/L50 mL2.5 mL

What Is the Weight/Volume Percent Dilution Calculator?

The Weight/Volume Percent Dilution Calculator is a tool that dilutes solutions expressed as grams of solute per 100 mL of solution (% w/v). A 10% w/v solution holds 10 g of solute in every 100 mL, which equals 100 g/L or 100 mg/mL.

% w/v is common in pharmacy, microbiology media, and biology buffers, such as 0.9% w/v sodium chloride (normal saline) and 10% w/v sodium dodecyl sulfate (SDS). The calculator returns the stock volume and states the g/L and mg/mL equivalents of each percent.

Common Uses

There are 5 main uses of this calculator.

  • Saline preparation

    Labs dilute 10% w/v NaCl to 0.9% w/v normal saline.

  • Detergent stocks

    Biochemists dilute 10% SDS or 10% Triton X-100 to 0.1% working levels.

  • Gel and stain components

    Users dilute 10% ammonium persulfate (APS) for gel casting.

  • Media supplements

    Microbiologists dilute 20% w/v sugar stocks into broth.

  • Pharmacy solutions

    Pharmacists dilute topical and oral solutions labeled in % w/v.

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