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DilutionCalculator
Concentration Units & Chemistry

Molarity Dilution Calculator

Dilute in M, mM, µM or nM. Mix units between stock and target.

Presets:
Formula: C₁ × V₁ = C₂ × V₂
V₁ = (C₂ × V₂) / C₁
Stock Source
Calculating
Target Conc
Final Volume
Fluid color:
Dilution Factor (DF)
5.00×
1 : 5 dilution
Solvent (Buffer / Water)
40.00 mL
V₂ - V₁ volume

Molarity Dilution Calculator: Live Flask Simulation

Live color sync
Transfer 10 mL aliquot
Stock Solution (C₁)
75%50%25%
10 mM
Concentration (C₁)
Aliquot to draw: 10 mL
Final Dilution (C₂)
CALIBRATION MARK (V₂)
2 mM
Concentration (C₂)
Total Volume: 50 mL
Color depth (Beer-Lambert) Relative Intensity: 20% (Absorbance Factor: 0.200)

Step-by-Step Bench Protocol

1

Pipette Stock Aliquot

Draw 10 mL of stock (10 mM) with a pipette.

2

Add Diluent / Buffer

Add 40 mL of water or buffer to reach the final mark.

3

Invert and Mix

Invert the tube to mix. You now have 50 mL at 2 mM (a 5× dilution).

How It Works

This calculator works in 4 steps.

  1. Enter the stock molarity

    Type the concentration in M, mM, µM, or nM.

  2. Enter the target molarity

    Type the working concentration; prefixes may differ from the stock.

  3. Enter the final volume

    Type the volume in L, mL, or µL.

  4. Read the stock volume and moles

    The calculator converts both concentrations to M, solves V₁ = M₂V₂ / M₁, and reports the moles of solute transferred.

The Formula

The formula sets the moles in the stock aliquot equal to the moles in the final solution.

Molar dilution M₁ × V₁ = M₂ × V₂
Moles transferred n = M₂ × V₂
Prefix scale 1 M = 10³ mM = 10⁶ µM = 10⁹ nM
M₁:
Stock molarity (mol/L)
M₂:
Target molarity
V₁ / V₂:
Stock and final volumes
n:
Moles of solute
Worked example

To make 500 mL of 1 M HCl from 6 M HCl, V₁ = 1 × 500 / 6 = 83.3 mL. The solution contains 0.5 mol (500 mmol) HCl.

Conversion Table

The conversion table lists stock volumes and moles transferred for 6 molar dilutions.

StockTargetFinal volumeStock volumeSolute amount
1 M0.1 M (100 mM)100 mL10 mL10 mmol
6 M1 M (1,000 mM)500 mL83.33 mL500 mmol
12.1 M0.5 M (500 mM)250 mL10.33 mL125 mmol
0.5 M0.05 M (50 mM)1,000 mL100 mL50 mmol
5 M0.15 M (150 mM)200 mL6 mL30 mmol
2 M0.002 M (2 mM)50 mL0.05 mL0.1 mmol

What Is the Molarity Dilution Calculator?

The Molarity Dilution Calculator is a tool that dilutes solutions expressed in moles per liter (mol/L, M) and returns the stock volume, diluent volume, and moles of solute transferred. The calculator accepts M, mM, µM, nM, and pM on either side and converts the prefixes before solving.

Molarity counts solute particles, so molar dilution keeps reaction stoichiometry visible. The number of moles in the stock aliquot equals the number of moles in the final solution: n = C × V. The calculator shows this mole count in mmol or µmol, so the user can match it with a reaction recipe.

Common Uses

There are 5 main uses of this calculator.

  • Acid and base preparation

    Chemists dilute concentrated HCl, H₂SO₄, and NaOH to working molarity.

  • Titrant preparation

    Analysts prepare 0.1 M and 0.01 M titrants from 1 M stocks.

  • Salt solutions

    Biologists make 150 mM NaCl from 5 M NaCl stock.

  • Reaction setup

    Researchers deliver an exact mole amount to a reaction vessel.

  • Stoichiometry teaching

    Instructors link dilution to mole counting in general chemistry.

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