How It Works
This calculator works in 4 steps.
Enter mass and MW
Type the supplied mass and the molecular weight from the certificate.
Choose the stock molarity
Type the stock strength, such as 10 mM, within the compound's stated solubility.
Enter the working concentration and volume
Type the assay concentration and volume from the protocol.
Read the volumes
The calculator returns solvent volume for the stock and stock volume for each working solution.
The Formula
The formula converts mass to moles, divides by the stock molarity, and applies C₁V₁ = C₂V₂ for working solutions.
V_solvent = m (mg) / MW × 10⁶ / C_stock (mM)V_add = C_work × V_work / C_stock- m:
- Compound mass
- MW:
- Molecular weight (g/mol)
- C_stock:
- Stock molarity
- C_work / V_work:
- Working concentration and volume
5 mg of a compound with MW 450.5 g/mol makes a 10 mM stock in 1,110 µL DMSO. 1 mL of 10 µM needs 1 µL of stock.
Conversion Table
The conversion table converts 5 compound amounts into stock solvent volume and working addition volume.
| Mass | MW | Stock | Solvent volume | Working target | Stock to add |
|---|---|---|---|---|---|
| 5 mg | 450.5 g/mol | 10 mM | 1,110 µL | 10 µM in 1 mL | 1 µL |
| 10 mg | 312.4 g/mol | 50 mM | 640.2 µL | 10 µM in 10 mL | 2 µL |
| 1 mg | 805 g/mol | 10 mM | 124.2 µL | 10 µM in 2 mL | 2 µL |
| 2 mg | 250.3 g/mol | 20 mM | 399.5 µL | 5 µM in 5 mL | 1.25 µL |
| 25 mg | 1,200 g/mol | 5 mM | 4,167 µL | 0.5 µM in 20 mL | 2 µL |
What Is the Research Compound Dilution Calculator?
The Research Compound Dilution Calculator is a tool that converts a weighed or supplied amount of a research compound into a molar stock and then into working concentrations for an experiment. The calculator links mass, molecular weight, stock molarity, and working molarity in one table.
Research compounds are often small-molecule inhibitors or agonists supplied as mg powders. The calculator returns the solvent volume for the stock and the stock volume per working dose. Doses and solvents come from the user's protocol; the tool does not suggest doses for use in people or animals.
Common Uses
There are 5 main uses of this calculator.
Inhibitor studies
Researchers prepare kinase inhibitor stocks and doses.
Agonist dosing
Pharmacologists set receptor agonist concentrations.
Screening stocks
Screening labs prepare 10 mM library stocks.
Solubility planning
Chemists choose stock strengths under solubility limits.
Inventory records
Labs log stock strength and volume per vial.