Skip to content
DilutionCalculator
Dilution Factors & Ratios

Dilution Ratio Calculator

Turn a mix ratio into real volumes. You set what the ratio means.

Presets:
Formula: C₁ × V₁ = C₂ × V₂
V₁ = (C₂ × V₂) / C₁
Stock Source
Calculating
Target Conc
Final Volume
Fluid color:
Dilution Factor (DF)
5.00×
1 : 5 dilution
Solvent (Buffer / Water)
40.00 mL
V₂ - V₁ volume

Dilution Ratio Calculator: Live Flask Simulation

Live color sync
Transfer 10 mL aliquot
Stock Solution (C₁)
75%50%25%
10 mM
Concentration (C₁)
Aliquot to draw: 10 mL
Final Dilution (C₂)
CALIBRATION MARK (V₂)
2 mM
Concentration (C₂)
Total Volume: 50 mL
Color depth (Beer-Lambert) Relative Intensity: 20% (Absorbance Factor: 0.200)

Step-by-Step Bench Protocol

1

Pipette Stock Aliquot

Draw 10 mL of stock (10 mM) with a pipette.

2

Add Diluent / Buffer

Add 40 mL of water or buffer to reach the final mark.

3

Invert and Mix

Invert the tube to mix. You now have 50 mL at 2 mM (a 5× dilution).

How It Works

This calculator works in 4 steps.

  1. Enter the ratio

    Type the 2 numbers, such as 1 and 4 for 1:4.

  2. Choose the convention

    Select concentrate-to-diluent (parts to parts) or concentrate-to-total (parts of the final mix).

  3. Enter the total volume

    Type the volume of finished solution, such as 1 L or 1 US gal (3.785 L).

  4. Read the concentrate and diluent volumes

    The calculator divides the total by the number of parts and multiplies by each part count.

The Formula

The formula divides the total volume by the sum of ratio parts and multiplies by each part.

Total parts (a:b, parts to parts) Parts = a + b
Concentrate volume V_conc = V_total × a / (a + b)
Diluent volume V_dil = V_total × b / (a + b)
a:
Concentrate parts
b:
Diluent parts
V_total:
Total volume of mix
V_conc / V_dil:
Concentrate and diluent volumes
Worked example

A 1:3 ratio for 1,000 mL makes 4 parts of 250 mL each: 250 mL concentrate and 750 mL water.

Conversion Table

The conversion table splits 7 common parts-to-parts ratios into concentrate and diluent volumes.

Ratio (conc:dil)Total partsTotal volumeConcentrateDiluent
1:12500 mL250 mL250 mL
1:341,000 mL250 mL750 mL
1:451,000 mL200 mL800 mL
1:910500 mL50 mL450 mL
1:15161,600 mL100 mL1,500 mL
1:32333,785 mL114.7 mL3,670 mL
1:64653,785 mL58.23 mL3,727 mL

3,785 mL = 1 US gallon. Ratios follow the concentrate-to-diluent convention.

What Is the Dilution Ratio Calculator?

The Dilution Ratio Calculator is a mixing tool that splits a total volume into concentrate and diluent portions according to an explicitly defined ratio, such as 1:4 (1 part concentrate to 4 parts water). The user states which convention the ratio follows before calculating, so the result does not depend on guesswork.

Dilution ratios appear on cleaning product labels, photographic chemistry, horticulture products, and lab protocols. A ratio of 1:4 in the parts-to-parts convention makes 5 total parts, so 1 L (33.8 US fl oz) of mix holds 200 mL of concentrate and 800 mL of water. The calculator outputs volumes in mL, L, US fl oz, and US gallons (gal).

Common Uses

There are 5 main uses of this calculator.

  • Product label instructions

    Users mix cleaning concentrates labeled 1:10, 1:32, or 1:64.

  • Photographic developers

    Darkroom users mix developers such as 1:1 and 1:9 stock-to-water dilutions.

  • Garden and plant products

    Gardeners mix liquid feed concentrates into watering cans.

  • Lab recipes in parts

    Technicians follow recipes written as parts, such as 1 part reagent to 2 parts water.

  • Batch scaling

    Users scale one ratio from a 500 mL spray bottle to a 5 US gal (18.9 L) bucket.

Related Tools in Dilution Factors & Ratios

View All →