How It Works
This calculator works in 4 steps.
Add each component
Type each liquid added and its volume.
Enter solvent content
Type the solvent name and its % v/v in each component.
Sum solvent volumes
The calculator multiplies each volume by its solvent fraction and sums by solvent.
Read the final composition
The calculator returns % v/v for each solvent and the total organic percent.
The Formula
The formula divides the summed solvent volume by the total mixture volume.
%S = 100 × Σ (Vᵢ × φᵢ) / Σ VᵢV_S,i = Vᵢ × φᵢ- Vᵢ:
- Volume of component i
- φᵢ:
- Solvent fraction in component i
- %S:
- Final solvent percent
Adding 10 µL DMSO stock to 990 µL medium gives 10 / 1,000 = 1.0% DMSO.
Conversion Table
The conversion table gives the final organic solvent percent for 5 mixtures.
| Components | Total volume | Solvent volume | Final solvent % |
|---|---|---|---|
| 10 µL DMSO stock + 990 µL Medium | 1,000 µL | 10 µL | 1% |
| 300 µL Methanol + 700 µL Water | 1,000 µL | 300 µL | 30% |
| 200 µL ACN stock 50% + 100 µL ACN + 700 µL Water | 1,000 µL | 200 µL | 20% |
| 50 µL Ethanol 95% + 950 µL Buffer | 1,000 µL | 47.5 µL | 4.75% |
| 5 µL DMSO stock + 5 µL Ethanol stock + 990 µL Medium | 1,000 µL | 10 µL | 1% |
What Is the Solvent Composition Calculator?
The Solvent Composition Calculator is a tool that tracks the percent of each organic solvent in a final mixture after one or more additions. The calculator sums the solvent volume carried by every component and divides by the total volume.
Organic solvents change protein stability, cell health, and chromatographic retention. Every addition of a DMSO, ethanol, or acetonitrile stock raises the solvent percent of the final mix. The calculator lists each solvent separately and reports the total organic percent in the final solution.
Common Uses
There are 5 main uses of this calculator.
Cell assays
Cell biologists keep DMSO under limits such as 0.1% to 0.5%.
HPLC mobile phases
Chromatographers set the organic percent of an eluent.
Protein work
Biochemists check organic content that affects folding.
Extraction
Analysts track solvent ratios in extraction mixtures.
Formulation
Formulators record solvent content in a product.