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Stock Reagent & Reconstitution

Concentrated Reagent Molarity Calculator

Find the M of a bottle acid from its label.

Presets:
Formula: C₁ × V₁ = C₂ × V₂
V₁ = (C₂ × V₂) / C₁
Stock Source
Calculating
Target Conc
Final Volume
Fluid color:
Dilution Factor (DF)
5.00×
1 : 5 dilution
Solvent (Buffer / Water)
40.00 mL
V₂ - V₁ volume

Concentrated Reagent Molarity Calculator: Live Flask Simulation

Live color sync
Transfer 10 mL aliquot
Stock Solution (C₁)
75%50%25%
10 mM
Concentration (C₁)
Aliquot to draw: 10 mL
Final Dilution (C₂)
CALIBRATION MARK (V₂)
2 mM
Concentration (C₂)
Total Volume: 50 mL
Color depth (Beer-Lambert) Relative Intensity: 20% (Absorbance Factor: 0.200)

Step-by-Step Bench Protocol

1

Pipette Stock Aliquot

Draw 10 mL of stock (10 mM) with a pipette.

2

Add Diluent / Buffer

Add 40 mL of water or buffer to reach the final mark.

3

Invert and Mix

Invert the tube to mix. You now have 50 mL at 2 mM (a 5× dilution).

How It Works

This calculator works in 4 steps.

  1. Enter the assay percent

    Type % w/w from the bottle or certificate.

  2. Enter the density

    Type the specific gravity or density in g/mL.

  3. Enter the molecular weight

    Type the MW of the active compound.

  4. Read the molarity

    The calculator returns M and the mL of concentrate per liter of 1 M solution.

The Formula

The formula multiplies density, mass percent, and 10, then divides by molecular weight.

Molarity M = (10 × ρ × w) / MW
Volume for 1 L of 1 M V (mL) = 1,000 / M
M:
Molarity (mol/L)
ρ:
Density (g/mL)
w:
Assay (% w/w)
MW:
Molecular weight (g/mol)
Worked example

98% sulfuric acid at 1.84 g/mL: M = 10 × 1.84 × 98 / 98.08 = 18.4 M. 1 L of 1 M H₂SO₄ needs 54.4 mL of concentrate.

Conversion Table

The conversion table lists the molarity of 7 common concentrated reagents and the volume per liter of 1 M solution.

ReagentAssayDensityMWMolaritymL per 1 L of 1 M
Hydrochloric acid (HCl)37%1.19 g/mL36.46 g/mol12.1 M82.8 mL
Sulfuric acid (H₂SO₄)98%1.84 g/mL98.08 g/mol18.4 M54.4 mL
Nitric acid (HNO₃)70%1.42 g/mL63.01 g/mol15.8 M63.4 mL
Acetic acid, glacial99.7%1.05 g/mL60.05 g/mol17.4 M57.4 mL
Phosphoric acid (H₃PO₄)85%1.69 g/mL98 g/mol14.7 M68.2 mL
Ammonium hydroxide (as NH₃)28%0.9 g/mL17.03 g/mol14.8 M67.6 mL
Hydrogen peroxide (H₂O₂)30%1.11 g/mL34.01 g/mol9.79 M102 mL

Values use typical label assay and density at 20 °C (68 °F). Check the lot certificate for exact figures.

What Is the Concentrated Reagent Molarity Calculator?

The Concentrated Reagent Molarity Calculator is a tool that returns the molarity of a concentrated liquid reagent from its assay percent (% w/w), density, and molecular weight. 37% hydrochloric acid with a density of 1.19 g/mL is 12.1 M.

Bottles of concentrated acids and bases list % w/w and density, not molarity. The molarity is the starting value for every dilution of that reagent. The calculator returns M and the volume of concentrate needed per liter of 1 M solution.

Common Uses

There are 5 main uses of this calculator.

  • Acid dilution planning

    Chemists compute V₁ for 1 M and 0.1 M acids.

  • Titrant preparation

    Analysts prepare standard acids before standardization.

  • Sample digestion

    Labs prepare nitric and hydrochloric acid digestion mixes.

  • Lot checks

    QC staff compare lots with different assay values.

  • Teaching

    Instructors show the link between % w/w, density, and M.

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